Aakash Marasini

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Researcher in a lab coat, mask and goggles examining an antimicrobial susceptibility test plate inside a biosafety cabinet

LET ME INTRODUCE MYSELF

I am a Nepal-registered veterinarian (BVSc & AH, Agriculture and Forestry University, CGPA 3.79) with a keen interest in microbiology and a passion for understanding how bacteria resist the drugs we rely on.

My work centres on antimicrobial resistance (AMR) in foodborne and zoonotic bacteria, including Salmonella, E. coli and Staphylococcus, across poultry, livestock, retail meat and wildlife in Nepal.

I enjoy the full path from sample collection and bacterial isolation to susceptibility testing, ESBL detection and biofilm assays, and I am keen to link phenotypic resistance with genotypic data from a One Health perspective.

I teach veterinary science at diploma level and enjoy mentoring students and collaborating in research teams.

Portrait of Aakash Marasini

RESEARCH INTERESTS

Antimicrobial resistance

CLSI-based susceptibility testing, MIC by broth microdilution, and ESBL (blaCTX-M) detection in animal and food isolates.

Molecular epidemiology

Linking phenotype to genotype, and tracing how resistant clones spread along the food chain.

Foodborne and zoonotic pathogens

Salmonella, E. coli and Staphylococcus across poultry, livestock, retail meat and wildlife.

Biofilms

Biofilm formation and antibiotic tolerance, from crystal-violet assays toward molecular mechanisms.

Surveillance and statistics

Epidemiological, spatial and statistical approaches, with data analysis and visualization in R.

One Health

Resistance across animals, the environment and people.

PUBLICATIONS

PublishedPoultry Science, 2025 · Q1
Molecular characterization, antimicrobial resistance profiling, and biofilm analysis of Salmonella isolates from dead-in-shell embryonated eggs
Khanal S, Luitel H, Adhikari S, Marasini A, Bhattarai RK. 104(11): 105773
doi.org/10.1016/j.psj.2025.105773
PublishedVeterinary and Animal Science, 2025 · Q1
Identification and multidrug resistance profiles of Escherichia coli, Salmonella, and Staphylococcus from cloacal swabs of captive yearling gharials (Gavialis gangeticus) in Chitwan National Park, Nepal
Adhikari S, Luitel H, Khanal S, Marasini A. 29: 100475
doi.org/10.1016/j.vas.2025.100475
PublishedVeterinary and Animal Science, 2025 · Q1
Antimicrobial resistance and biofilm-forming ability in Staphylococcus aureus causing clinical bovine mastitis in Chitwan, Nepal
Adhikari S, Khanal S, Marasini A, Panthi P, Adhikari A, Luitel H, Pandeya YR. 30: 100508
doi.org/10.1016/j.vas.2025.100508
PublishedJournal of Food Protection, 2026 · Q2
Prevalence and antimicrobial resistance profiles of Salmonella and Escherichia coli, and hygiene practices in retail poultry meat shops of Bharatpur, Nepal
Thapa S, Luitel H, Adhikari S, Khanal S, Marasini A, Thapa S. 100906
doi.org/10.1016/j.jfp.2026.100906
PublishedJournal of Food Protection, 2026 · Q2
Tissue-site distribution, antimicrobial resistance profile, and ESBL production of Escherichia coli isolated from retail poultry carcasses in Bharatpur Metropolitan City, Chitwan, Nepal
Corresponding author. 100955
doi.org/10.1016/j.jfp.2026.100955

UNDER REVIEW

🔒 To see the under-review papers, please contact aakash.marasini@favf.edu.np
Under review
Journal of Applied Poultry Research · JAPR-D-26-00234
Isolation, antibiogram, biofilm-forming ability, and molecular detection of blaCTX-M gene among ESBL-producing Escherichia coli isolates from dead-in-shell chicken embryos
First author and principal investigator
Under review
J. Preventive Medicine: Regional Studies and Reports · PVMRSR-D-26-00195
Bacterial isolation and antimicrobial susceptibility profile of Staphylococcus aureus and Escherichia coli from foot lesions in captive Asian elephants of Chitwan, Nepal
Corresponding author
Under review
J. Preventive Medicine: Regional Studies and Reports · PVMRSR-D-26-00202
Gastrointestinal helminthiasis in large ruminants: prevalence and risk factor analysis in farms of Gorkha Municipality, Nepal
Co-author

Full record on ORCID

EXPERIENCE & EDUCATION

Veterinary Instructor

Dhaulagiri Polytechnic Institute (CTEVT), Baglung · Jan 2026 – Present

Teaching pharmacology, veterinary microbiology and parasitology, clinical medicine and animal husbandry to diploma-level students.

Veterinary Officer (6th Level) and Section Head

Musikot Municipality, Gulmi · Apr 2025 – Jan 2026

Led municipal veterinary services: clinical case management, disease surveillance, vaccination campaigns, outbreak reporting and farmer extension. Supervised junior staff and coordinated disease reporting with provincial livestock authorities.

BVSc & AH, Agriculture and Forestry University

Rampur, Chitwan · 2019 – 2025 · CGPA 3.79

Thesis on blaCTX-M ESBL-producing E. coli from dead-in-shell chicken embryos, supervised by Prof. Rebanta Kumar Bhattarai, PhD.

Registered Veterinarian, Nepal Veterinary Council

Permanent registration

Winner, Nepal Young Bioentrepreneurs Competition 2023, supported by USAID.

RESEARCH SKILLS

Microbiology

Isolation, identification and phenotypic characterization of E. coli, Salmonella and S. aureus.

AMR assays

CLSI disc diffusion, broth microdilution (MIC), ESBL phenotypic confirmation.

Biofilm assays

Crystal-violet microtiter plate assay, Congo red agar and tube methods.

Molecular techniques

DNA extraction and quantification, conventional PCR, resistance and virulence gene detection, gel electrophoresis.

Data analysis

Statistical analysis and visualization in R.

Research practice

Study design, sample collection, scientific writing and manuscript preparation.

GET IN TOUCH

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Location

Resunga-06, Gulmi, Nepal

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aksmarasini21